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esr1 rnascope probe  (Advanced Cell Diagnostics Inc)

 
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    Advanced Cell Diagnostics Inc esr1 rnascope probe
    a, <t>RNAscope</t> staining of Esr1, Vglut2 and Vgat in MPOA. Scale bar, 50um. b, Percentage of neurons in different specific categories. 80% of Esr1+ neurons were Vgat+. c, Dual-color CTb retrograde tracing of MPOA neurons projecting to VTA and PAG in Esr1-Cre::Ai14 mice. d, Images in MPOA. Arrows indicate Esr1+ (tdTomato+) neurons co-labelled with CTb647 (blue, from PAG) or CTb488 (green, from VTA). Scale bar, 100μm. e, Percentage of neurons in specific categories. 60% of Esr1+ neurons were labeled by either CTB647 or CTb488. Mean ± s.d., n = 3 animals. f, Average half-peak spike width of MPOA Vgat+ neurons for spikes evoked by current injections in NHW and HW slices. n=14 cells for NHW group and 12 cells for HW group, from 3 animals in each group. ****P < 0.0001, two-tailed Mann-Whitney test. g, Average trough voltage at different injection current amplitudes. n = 6, 6 cells for NHW and HW group, from 3 animals respectively. *P < 0.05, **P < 0.01, ***P < 0.001, two-way repeated measures ANOVA. h, Average half-peak spike width of spontaneous spikes of MPOA Esr1+ neurons in NHW (n=10 cells from 3 animals) and HW (n=10 cells from 3 animals) slices. ***P = 0.0002, two-tailed Mann-Whitney test. i, Average trough voltage of spontaneous spikes. n=10,10 cells for NHW and HW group, from 3 animals for each group. ***P = 0.0002, two-tailed Mann-Whitney test. For boxplot, centerline, mean, upper and lower end, 90 and 10 percentiles.
    Esr1 Rnascope Probe, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/esr1+rnascope+probe/pmc11037266-636-21-17?v=Advanced+Cell+Diagnostics+Inc
    Average 90 stars, based on 1 article reviews
    esr1 rnascope probe - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "The medial preoptic area mediates depressive-like behaviors induced by ovarian hormone withdrawal through distinct GABAergic projections"

    Article Title: The medial preoptic area mediates depressive-like behaviors induced by ovarian hormone withdrawal through distinct GABAergic projections

    Journal: Nature neuroscience

    doi: 10.1038/s41593-023-01397-2

    a, RNAscope staining of Esr1, Vglut2 and Vgat in MPOA. Scale bar, 50um. b, Percentage of neurons in different specific categories. 80% of Esr1+ neurons were Vgat+. c, Dual-color CTb retrograde tracing of MPOA neurons projecting to VTA and PAG in Esr1-Cre::Ai14 mice. d, Images in MPOA. Arrows indicate Esr1+ (tdTomato+) neurons co-labelled with CTb647 (blue, from PAG) or CTb488 (green, from VTA). Scale bar, 100μm. e, Percentage of neurons in specific categories. 60% of Esr1+ neurons were labeled by either CTB647 or CTb488. Mean ± s.d., n = 3 animals. f, Average half-peak spike width of MPOA Vgat+ neurons for spikes evoked by current injections in NHW and HW slices. n=14 cells for NHW group and 12 cells for HW group, from 3 animals in each group. ****P < 0.0001, two-tailed Mann-Whitney test. g, Average trough voltage at different injection current amplitudes. n = 6, 6 cells for NHW and HW group, from 3 animals respectively. *P < 0.05, **P < 0.01, ***P < 0.001, two-way repeated measures ANOVA. h, Average half-peak spike width of spontaneous spikes of MPOA Esr1+ neurons in NHW (n=10 cells from 3 animals) and HW (n=10 cells from 3 animals) slices. ***P = 0.0002, two-tailed Mann-Whitney test. i, Average trough voltage of spontaneous spikes. n=10,10 cells for NHW and HW group, from 3 animals for each group. ***P = 0.0002, two-tailed Mann-Whitney test. For boxplot, centerline, mean, upper and lower end, 90 and 10 percentiles.
    Figure Legend Snippet: a, RNAscope staining of Esr1, Vglut2 and Vgat in MPOA. Scale bar, 50um. b, Percentage of neurons in different specific categories. 80% of Esr1+ neurons were Vgat+. c, Dual-color CTb retrograde tracing of MPOA neurons projecting to VTA and PAG in Esr1-Cre::Ai14 mice. d, Images in MPOA. Arrows indicate Esr1+ (tdTomato+) neurons co-labelled with CTb647 (blue, from PAG) or CTb488 (green, from VTA). Scale bar, 100μm. e, Percentage of neurons in specific categories. 60% of Esr1+ neurons were labeled by either CTB647 or CTb488. Mean ± s.d., n = 3 animals. f, Average half-peak spike width of MPOA Vgat+ neurons for spikes evoked by current injections in NHW and HW slices. n=14 cells for NHW group and 12 cells for HW group, from 3 animals in each group. ****P < 0.0001, two-tailed Mann-Whitney test. g, Average trough voltage at different injection current amplitudes. n = 6, 6 cells for NHW and HW group, from 3 animals respectively. *P < 0.05, **P < 0.01, ***P < 0.001, two-way repeated measures ANOVA. h, Average half-peak spike width of spontaneous spikes of MPOA Esr1+ neurons in NHW (n=10 cells from 3 animals) and HW (n=10 cells from 3 animals) slices. ***P = 0.0002, two-tailed Mann-Whitney test. i, Average trough voltage of spontaneous spikes. n=10,10 cells for NHW and HW group, from 3 animals for each group. ***P = 0.0002, two-tailed Mann-Whitney test. For boxplot, centerline, mean, upper and lower end, 90 and 10 percentiles.

    Techniques Used: RNAscope, Staining, Retrograde Tracing, Labeling, Two Tailed Test, MANN-WHITNEY, Injection



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    a, <t>RNAscope</t> staining of Esr1, Vglut2 and Vgat in MPOA. Scale bar, 50um. b, Percentage of neurons in different specific categories. 80% of Esr1+ neurons were Vgat+. c, Dual-color CTb retrograde tracing of MPOA neurons projecting to VTA and PAG in Esr1-Cre::Ai14 mice. d, Images in MPOA. Arrows indicate Esr1+ (tdTomato+) neurons co-labelled with CTb647 (blue, from PAG) or CTb488 (green, from VTA). Scale bar, 100μm. e, Percentage of neurons in specific categories. 60% of Esr1+ neurons were labeled by either CTB647 or CTb488. Mean ± s.d., n = 3 animals. f, Average half-peak spike width of MPOA Vgat+ neurons for spikes evoked by current injections in NHW and HW slices. n=14 cells for NHW group and 12 cells for HW group, from 3 animals in each group. ****P < 0.0001, two-tailed Mann-Whitney test. g, Average trough voltage at different injection current amplitudes. n = 6, 6 cells for NHW and HW group, from 3 animals respectively. *P < 0.05, **P < 0.01, ***P < 0.001, two-way repeated measures ANOVA. h, Average half-peak spike width of spontaneous spikes of MPOA Esr1+ neurons in NHW (n=10 cells from 3 animals) and HW (n=10 cells from 3 animals) slices. ***P = 0.0002, two-tailed Mann-Whitney test. i, Average trough voltage of spontaneous spikes. n=10,10 cells for NHW and HW group, from 3 animals for each group. ***P = 0.0002, two-tailed Mann-Whitney test. For boxplot, centerline, mean, upper and lower end, 90 and 10 percentiles.
    Esr1 Rnascope Probe, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a, <t>RNAscope</t> staining of Esr1, Vglut2 and Vgat in MPOA. Scale bar, 50um. b, Percentage of neurons in different specific categories. 80% of Esr1+ neurons were Vgat+. c, Dual-color CTb retrograde tracing of MPOA neurons projecting to VTA and PAG in Esr1-Cre::Ai14 mice. d, Images in MPOA. Arrows indicate Esr1+ (tdTomato+) neurons co-labelled with CTb647 (blue, from PAG) or CTb488 (green, from VTA). Scale bar, 100μm. e, Percentage of neurons in specific categories. 60% of Esr1+ neurons were labeled by either CTB647 or CTb488. Mean ± s.d., n = 3 animals. f, Average half-peak spike width of MPOA Vgat+ neurons for spikes evoked by current injections in NHW and HW slices. n=14 cells for NHW group and 12 cells for HW group, from 3 animals in each group. ****P < 0.0001, two-tailed Mann-Whitney test. g, Average trough voltage at different injection current amplitudes. n = 6, 6 cells for NHW and HW group, from 3 animals respectively. *P < 0.05, **P < 0.01, ***P < 0.001, two-way repeated measures ANOVA. h, Average half-peak spike width of spontaneous spikes of MPOA Esr1+ neurons in NHW (n=10 cells from 3 animals) and HW (n=10 cells from 3 animals) slices. ***P = 0.0002, two-tailed Mann-Whitney test. i, Average trough voltage of spontaneous spikes. n=10,10 cells for NHW and HW group, from 3 animals for each group. ***P = 0.0002, two-tailed Mann-Whitney test. For boxplot, centerline, mean, upper and lower end, 90 and 10 percentiles.
    Esr1 Rnascope Probe Mix, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    a, RNAscope staining of Esr1, Vglut2 and Vgat in MPOA. Scale bar, 50um. b, Percentage of neurons in different specific categories. 80% of Esr1+ neurons were Vgat+. c, Dual-color CTb retrograde tracing of MPOA neurons projecting to VTA and PAG in Esr1-Cre::Ai14 mice. d, Images in MPOA. Arrows indicate Esr1+ (tdTomato+) neurons co-labelled with CTb647 (blue, from PAG) or CTb488 (green, from VTA). Scale bar, 100μm. e, Percentage of neurons in specific categories. 60% of Esr1+ neurons were labeled by either CTB647 or CTb488. Mean ± s.d., n = 3 animals. f, Average half-peak spike width of MPOA Vgat+ neurons for spikes evoked by current injections in NHW and HW slices. n=14 cells for NHW group and 12 cells for HW group, from 3 animals in each group. ****P < 0.0001, two-tailed Mann-Whitney test. g, Average trough voltage at different injection current amplitudes. n = 6, 6 cells for NHW and HW group, from 3 animals respectively. *P < 0.05, **P < 0.01, ***P < 0.001, two-way repeated measures ANOVA. h, Average half-peak spike width of spontaneous spikes of MPOA Esr1+ neurons in NHW (n=10 cells from 3 animals) and HW (n=10 cells from 3 animals) slices. ***P = 0.0002, two-tailed Mann-Whitney test. i, Average trough voltage of spontaneous spikes. n=10,10 cells for NHW and HW group, from 3 animals for each group. ***P = 0.0002, two-tailed Mann-Whitney test. For boxplot, centerline, mean, upper and lower end, 90 and 10 percentiles.

    Journal: Nature neuroscience

    Article Title: The medial preoptic area mediates depressive-like behaviors induced by ovarian hormone withdrawal through distinct GABAergic projections

    doi: 10.1038/s41593-023-01397-2

    Figure Lengend Snippet: a, RNAscope staining of Esr1, Vglut2 and Vgat in MPOA. Scale bar, 50um. b, Percentage of neurons in different specific categories. 80% of Esr1+ neurons were Vgat+. c, Dual-color CTb retrograde tracing of MPOA neurons projecting to VTA and PAG in Esr1-Cre::Ai14 mice. d, Images in MPOA. Arrows indicate Esr1+ (tdTomato+) neurons co-labelled with CTb647 (blue, from PAG) or CTb488 (green, from VTA). Scale bar, 100μm. e, Percentage of neurons in specific categories. 60% of Esr1+ neurons were labeled by either CTB647 or CTb488. Mean ± s.d., n = 3 animals. f, Average half-peak spike width of MPOA Vgat+ neurons for spikes evoked by current injections in NHW and HW slices. n=14 cells for NHW group and 12 cells for HW group, from 3 animals in each group. ****P < 0.0001, two-tailed Mann-Whitney test. g, Average trough voltage at different injection current amplitudes. n = 6, 6 cells for NHW and HW group, from 3 animals respectively. *P < 0.05, **P < 0.01, ***P < 0.001, two-way repeated measures ANOVA. h, Average half-peak spike width of spontaneous spikes of MPOA Esr1+ neurons in NHW (n=10 cells from 3 animals) and HW (n=10 cells from 3 animals) slices. ***P = 0.0002, two-tailed Mann-Whitney test. i, Average trough voltage of spontaneous spikes. n=10,10 cells for NHW and HW group, from 3 animals for each group. ***P = 0.0002, two-tailed Mann-Whitney test. For boxplot, centerline, mean, upper and lower end, 90 and 10 percentiles.

    Article Snippet: Briefly, brain sections were hybridized with Esr1 (Advanced Cell Diagnostics, 478201), Vglut2 (Advanced Cell Diagnostics, 319171), Vgat (Advanced Cell Diagnostics, 319191) RNAscope probe mix, which were designed and validated by Adcanced Cell Diagostics.

    Techniques: RNAscope, Staining, Retrograde Tracing, Labeling, Two Tailed Test, MANN-WHITNEY, Injection